• The top concern for the

  2021-11-06

  

  The top concern for the clinical use of CRISPR-Cas9 technology is safety. Off-target alterations must be avoided to ensure genome integrity and proper cellular function. WGS found no off-target effects among the top 49 predicted off-target sites. WGS did find 4,658 indels and 1,891 SNVs in sgRNA1/2-

• In our study we evaluated adenovirus type Ad as a

  2021-11-06

  

  In our study we evaluated adenovirus type 5 (Ad5) as a vehicle for delivering Cre recombinase in conditional transgenic mouse based on the Cre-loxP system, in order to achieve GlyT1 gene modulation in a specific thalamic region in the adult animal. GlyT1 is a sodium- and chloride-coupled glycine tra

• br Acknowledgements This work was supported by the

  2021-11-06

  

  Acknowledgements This work was supported by the Science and Technology Department of Hubei Province (No.2016CFB368) and the National Natural Science Foundation of China (No. 81201053). Introduction L-Glutamate (glutamate) is the most important excitatory neurotransmitter of the central nervou

• A key limiting factor in the semi quantitative analysis

  2021-11-06

  

  A key limiting factor in the semi-quantitative analysis of changes in the nuclear-to-cytoplasmic distribution of glucokinase is the large intercellular heterogeneity of Naproxen Sodium of glucokinase in both isolated hepatocytes in vitro and also in liver in vivo[13], [23], [24], [26], [36]. This ne

• It is increasingly argued that the immunosuppressive effects

  2021-11-06

  

  It is increasingly argued that the immunosuppressive effects of GCs are conferred indirectly by GR through the activation of genes encoding proteins that inhibit Tanshinone IIA---sulfonic sodium of proinflammatory genes 86, 87, 88. Genomic studies offer another mechanism compatible with the idea th

• br Material and methods br

  2021-11-06

  

  Material and methods Results Discussion Accumulating evidence suggests that the hippocampus is sensitive to noise-induced hearing loss and other forms of sensory deprivation, which deprives the hippocampus of sensory information required for its normal operations such as spatial navigation

• In the a first in vitro indication of modulation

  2021-11-06

  

  In the 2003, a first in vitro indication of modulation of FXR expression in CRC showed that FXR expression was absent in undifferentiated colon carcinoma cell line SW480, while it was progressively increasing along with the degree of cell differentiation in Caco2 and HT29 cells, and its transcriptio

• In stark contrast to this study Zhang

  2021-11-06

  

  In stark contrast to this study, Zhang and colleagues demonstrated that FFA4, along with FFA1, play roles in suppressing cell proliferation and promoting apoptosis in CRC Thymidine australia treated with omega-3 PUFA [53]. In this study design, in vivo CRC was induced in mice upon treatment with th

• This hypothesis is in line with our phylogenetic

  2021-11-06

  

  This hypothesis is in line with our phylogenetic analysis of FBPase sequences which revealed that vertebrate FBP2 evolved significantly faster than FBP1 (Fig. 2). An average distance between FBP2 and ancestral vertebrate FBPase was higher than that for FBP1. Moreover, preliminary study on amphibia

• Several other assay parameters were also explored

  2021-11-06

  

  Several other assay parameters were also explored. First, the thermal dependence of the assay was investigated by measuring the specific activity at different temperatures (Fig. 7). That data show that enzymatic activity increases with increasing temperature. Interestingly, the observed rate increas

• To address this we developed approaches

  2021-11-06

  

  To address this, we developed approaches to image at high spatial and temporal resolution live WPB fusions as well as the release of VWF and other WPB cargo by spinning disk microscopy and combined laser ablation and total internal reflection fluorescence (TIRF) microscopy. Our live cell imaging rev

• Based on the inhibitory potency of C for HDAC

  2021-11-06

  

  Based on the inhibitory potency of C646 for HDAC6, we monitored α-tubulin acetylation; an HDAC6 substrate. C646 treatment provided pronounced inhibition of α-tubulin acetylation after both 6 and 20h of incubation, which argues against HDAC6 inhibition by C646 in RAW264.7 cells. The observed effect o

• The CRY protein contains a conserved photolyase homology reg

  2021-11-06

  

  The CRY1 protein contains a conserved photolyase homology region crucially involved in repression of CLOCK/BMAL1; a C-terminal helix also known as the predicted coiled coil (CC), which interacts with PER2 and FBXL3 in a mutually exclusive manner; and a C-terminal extension also referred to as the “t

• Cut proteins have five evolutionarily conserved domains

  2021-11-06

  

  Cut proteins have five evolutionarily conserved domains. These include a homeodomain, three Cut repeats, and a coiled coil structure. The cut repeats, called CR1, CR2, and CR3, are composed of 70 amino acids, and, along with the homeodomain, are each capable of binding DNA. Mammalian cut proteins fu

• A repertoire of cytotoxic payloads

  2021-11-05

  

  A repertoire of cytotoxic payloads is increasing; among those, MMAE has been utilized for the highly effective ADC brentuximab vedotin. However, the ADCs which utilized MMAE to target GCC in solid tumors have shown a poor efficacy suggesting that various tumor types respond differently to a given to

16040 records 576/1070 page Previous Next First page 上5页 576577578579580 下5页 Last page