• In our study caspase and DRAM were

  2023-12-25

  

  In our study, caspase3 and DRAM were identified as being involved in full-length AIFM1-induced apoptosis. Caspase3 is best known for its role in the execution phase of apoptosis in both intrinsic and extrinsic apoptotic pathways [17]. The members of the caspase family are generally in inactive pro-f

• To investigate if WP increased CAT

  2023-12-25

  

  To investigate if WP increased CAT activity in cells, C2C12 muscle conotoxin were treated with 0.1 to 0.4 mg/mL of WP (80.05% protein) for 24 h and then stressed with 0.75 mM H2O2 for 1 h (Xu et al., 2011). The CAT activity was significantly enhanced from 15.1 ± 0.7 to 23.7 ± 1.3 U/mg of total cell

• br Results br Discussion By using an unbiased proteomic

  2023-12-22

  

  Results Discussion By using an unbiased proteomic screen for xCT binding partners, followed by functional validation, we have made the surprising discovery that mTORC2 regulates amino sirtuins metabolism in tumor cells by phosphorylating serine 26 of the cystine-glutamate antiporter xCT on it

• br Materials and methods br Results br Discussion

  2023-12-22

  

  Materials and methods Results Discussion Knowledge of the molecular basis of an inherited genetic disease is crucial for understanding the disease pathology and, ultimately, for therapeutic design. Several recent reports have stressed the need for physical characterization of disease-associ

• Studies on both TCDD treated

  2023-12-22

  

  Studies on both TCDD treated mice and AhR null mice have also pointed to a role of AhR in hematopoiesis [65], [66]. Singh et al. showed that AhR is a negative regulator of HSC (hematopoietic stem cell) proliferation, while deletion of AhR leads to spleen enlargement in juvenile and adult mice [67].

• thymidylate synthase inhibitor br Conclusions br Acknowledgm

  2023-12-22

  

  Conclusions Acknowledgments The authors are thankful to Hugo Santos, Olga Martinez and Ricardo Lacerda for the technical assistance provided in the maintenance of the aquaria. This work was supported by national funds, through FCT and co-funded by the European Regional Development Fund (ERDF)

• Fig A shows a schematic representation of histological resul

  2023-12-22

  

  Fig. 2A shows a schematic representation of histological results according to the Franklin and Paxinos Mouse Brain Atlas (2001). The black circles represent the sites of drug infusion that were on-target within the amygdala. Gray circles represent the animals that had infusion locations outside the

• br Conclusion br Conflicts of interest br

  2023-12-22

  

  Conclusion Conflicts of interest Introduction RNA interference is a specific post-transcriptional gene silencing mechanism within a cell via the transfection of microRNAs (miRNA), exogenous small interfering RNAs (siRNA) or small hairpin RNAs (shRNA) [1–3]. Transfection of shRNA can be done

• The identification of novel kinase inhibitor scaffolds is hi

  2023-12-22

  

  The identification of novel kinase inhibitor scaffolds is highly desirable in order to develop selective kinase inhibitors. Small-molecule inhibitors of Interleukin-2-inducible T-cell kinase (Itk) that are based on the 3-aminopyridin-2-one fragment 1 have been reported. Despite derivatisation of 1 y

• br Conflicts of interest br Introduction Phosphatidic acid p

  2023-12-22

  

  Conflicts of interest Introduction Phosphatidic DIDS phosphatase (PAP) enzymes are responsible for catalyzing the reaction that dephosphorylates phosphatidic acid (PA), which in turn produces diacylglycerol (DAG) and a phosphate group during phospholipid regulation (Fig. 1A) [1]. Subsequently

• Acl converts cytosolic citrate CoA and ATP into acetyl CoA

  2023-12-22

  

  Acl converts cytosolic citrate, CoA and ATP into acetyl-CoA, ADP+Pi and oxaloacetate (Fig. 1). This cytosolic enzyme is present in few Prokaryotes and in all Eukaryotes, but not in non-oleaginous yeasts [14]. Thus, this enzyme was presumed to be essential for FA synthesis [1], [13], [15]. In most mi

• br Colorectal carcinoma The growth rate

  2023-12-22

  

  Colorectal carcinoma The growth rate of murine colon adenocarcinoma tumors decreased upon inhibition of 5-LOX, an enzyme that harbors counteracting properties to 15-LOX [28]. It is not clear whether 15-LOX was associated with this phenomenon, but its expression is induced in the colon carcinoma c

• Another possible explanation of the increased BOP levels

  2023-12-22

  

  Another possible explanation of the increased BOP levels might be excess cement. In the present study, cement-retained fixed restorations were used. Cementation of the implant-supported restorations may cause the insufficient removal of excess cement from the peri-implant sulcus. Also, the placement

• TUNEL assay Apoptotic DNA fragmentation in cells

  2023-12-22

  

  TUNEL assay. Apoptotic DNA fragmentation in ARQ 197 was measured using a commercially available TUNEL assay kit (Thermo Fisher). Frozen cells were fixed with 10% paraformaldehyde/PBS. Apoptosis was determined by staining the 3′-OH ends of fragmented DNA with biotin-dNTP using DNA I klenow fragment

• br Antioxidant polymers conjugates Natural antioxidants are

  2023-12-21

  

  Antioxidant-polymers conjugates Natural antioxidants are usually used as a substitute for synthetics but some degradation phenomena could influence their applications. For example, ascorbic oicr australia as a natural antioxidant undergoes yellowish coloration as a result of oxidation [29]. Sing

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